ABSTRACT

Background: The seroprevalence and risk factors for Toxoplasma gondii infection were examined among high-risk groups in

Gharyan, Libya—a semi-arid region with limited prior data. Aim: Given the severe health risks of toxoplasmosis in

immunocompromised individuals and pregnant women, and the scarcity of studies from western Libya, this research aimed to

address a key knowledge gap while comparing results with those from humid coastal cities.

Methods: A cross-sectional study was conducted from October 2022 to March 2023, involving 45 blood donors, 40

hemodialysis patients, and 146 pregnant women. A validated questionnaire was administered, and an

electrochemiluminescence immunoassay (ECLIA) was used to detect anti-Toxoplasma gondii IgG/IgM antibodies.

Results: Results revealed an overall higher seroprevalence among hemodialysis patients (40.0%), followed by pregnant women

(24.7%), and then blood donors (15.6%). Active (IgM+) infections were rare. Significant risk factors among pregnant women

included consumption of unwashed vegetables, undercooked meat, cat ownership, and rural residence (Adjusted Odds Ratio

aOR=3.42, aOR=3.15, aOR=2.87, aOR=2.05, respectively). No statistically significant difference in seroprevalence was

observed among pregnant women between 2022 (26.2%) and 2023 (23.5%) (P = 0.68).

Conclusion: A lower seroprevalence was observed in Gharyan municipality compared to coastal Libyan cities, indicating the

influence of the dry climate. The study recommends routine first-trimester screening for pregnant women (especially aged 25–

30 years), mandatory pre-dialysis testing for hemodialysis patients, and targeted food safety education focusing on vegetable

washing and thorough meat cooking.

Keywords: Toxoplasma gondii, Seroprevalence, , Pregnant Women, Blood Donors s