Antimicrobial Susceptibility Testing (AST) is essential for guiding appropriate antibiotic therapy. The disk diffusion method is widely used in Libya due to its low cost, yet deviations from standardized protocols can compromise result accuracy, affecting clinical decisions and patient outcomes. This study evaluated the performance of the disk diffusion AST method in medical laboratories in Gharyan, Libya, using EUCAST guidelines (version 12.0) as a benchmark. Seventy-five AST plates representing 122 clinical specimens were randomly collected from three pathology centers between September and November 2025. Plates were analyzed for the type of culture media, number of specimens per plate, number of antibiotic disks, and readability of results. All plates were 90 mm in diameter. Nutrient agar was used for 118 specimens, and blood agar for two; none used Mueller-Hinton agar as recommended. Only 17% of plates tested a single specimen per plate; the majority tested one specimen on half or a quarter of a plate. The number of antibiotic disks per specimen often exceeded EUCAST limits (up to 20 disks per plate), and only 8% of plates yielded readable results. Widespread deviations from EUCAST protocols—including inappropriate media, specimen overloading, and excessive disks—compromise AST reliability. These errors may lead to incorrect antibiotic prescriptions, delayed recovery, and increased antimicrobial resistance. Strict adherence to standardized guidelines is urgently required to ensure accurate, clinically meaningful AST results. Moreover, raising awareness of the importance of following standard guidelines is crucial for correcting the course

Abstract

The proper management of biological waste in laboratory settings is a critical aspect of biosafety in microbiological and biomedical laboratories. Beyond protecting lab workers, biological waste management safeguards public health, protects the environment, and ensures compliance with legal and regulatory frameworks. Segregation, decontamination of liquid waste, decontamination of solid waste, and techniques for inactivating toxins are all main aspects of biological waste management that will be outlined, aligned, and summarized with the main institutional biosafety protocols for biological waste management, published by major authorities, including CDC and WHO. This research found that the information from the two institutions complements each other. Therefore, this brief review aimed to compile and unify information from both institutions to construct a single, coherent text that provides a practical summary for medical laboratory professionals seeking to enhance the implementation of international, harmonized protocols that strengthen biosafety, environmental stewardship, and regulatory compliance

Abstract INTRODUCTION: Dental caries is a major global issue that has been connected to a number of systemic issues, necessitating multidisciplinary investigation. Examining dental degradation in conjunction with Helicobacter pylori infection provides a unique perspective on gut and oral health in Tripoli, Libya. Previously linked to peptic ulcers and chronic gastritis, H. pylori has recently been linked to tooth health. This change in viewpoint is crucial because there was no discernible link between the infection and chronic illnesses like diabetes, hypertension, or even smoking, indicating the necessity for measures to raise awareness of good oral hygiene among afflicted individuals. The prevalence of H. pylori was clearly linked to declining dental health when examining Decayed, Missing, and Filled Teeth (DMFT) scores; this relationship was particularly evident in older participants and those with poor oral hygiene. METHODS AND MATERIALS: a cross-sectional design was used, 135 randomly selected participants to ensure that our sample was representative of the local community. Following a thorough review of each patient's medical history, which included chronic conditions, socioeconomic status, and dental hygiene practices, a dentist qualified to perform this type of evaluation examined each patient for evidence of decay using well-known metrics such as the Decayed, Missing, and Filled Teeth (DMFT) score. Based on previous research, serological tests (detection of H. pylori antibodies) were performed to determine the presence of H. pylori in order to minimize discomfort and avoid the bias that occasionally arises with more intrusive gut collection methods. THE AIM: of this study is to provide a significant contribution to the present literature with genuine potential to influence public health policies and dental care programs targeted at reducing health inequities in the region, provided that the study is carefully planned and some innovative data management is done. RESULTS: This result essentially shows that additional interdisciplinary research is required to adequately map out the cause-and-effect relationship between H. pylori and dental caries. Curiously, it also supports previous research that suggested this bacterium might contribute to oral disorders, supporting the notion that a comprehensive, multimodal strategy is required to address oral and general health concerns. By lowering the rate of dental decay, local, community-driven initiatives to enhance oral hygiene may hold the key to minimizing the effects of H. pylori. The study emphasizes that reducing the disparities in oral health observed in Tripoli may depend on making dental treatment more accessible, particularly for those from lower-income backgrounds. Conclusion: An infection with Helicobacter pylori is substantially linked to a higher prevalence of dental caries. Both dental decay and H. pylori infection are significantly influenced by age, with poorer oral health being seen in older people. Infrequent dental checkups and poor oral hygiene are two factors that raise the DMFT index. People with diabetes, high blood pressure, and acidity have far worse tooth health. Dental caries is significantly influenced by economic level, with slightly worse outcomes for those with greater incomes. The DMFT score and H. pylori prevalence do not seem to be significantly impacted by smoking or gender. This study confirms our presumptions between H. pylori and dental cavities while highlighting the critical necessity for integrated healthcare systems. Keywords: Dental Caries, Helicobacter Pylori, Gastritis, Tripoli, Libya.

Abstract INTRODUCTION: Dental caries is a major global issue that has been connected to a number of systemic issues, necessitating multidisciplinary investigation. Examining dental degradation in conjunction with Helicobacter pylori infection provides a unique perspective on gut and oral health in Tripoli, Libya. Previously linked to peptic ulcers and chronic gastritis, H. pylori has recently been linked to tooth health. This change in viewpoint is crucial because there was no discernible link between the infection and chronic illnesses like diabetes, hypertension, or even smoking, indicating the necessity for measures to raise awareness of good oral hygiene among afflicted individuals. The prevalence of H. pylori was clearly linked to declining dental health when examining Decayed, Missing, and Filled Teeth (DMFT) scores; this relationship was particularly evident in older participants and those with poor oral hygiene. METHODS AND MATERIALS: a cross-sectional design was used, 135 randomly selected participants to ensure that our sample was representative of the local community. Following a thorough review of each patient's medical history, which included chronic conditions, socioeconomic status, and dental hygiene practices, a dentist qualified to perform this type of evaluation examined each patient for evidence of decay using well-known metrics such as the Decayed, Missing, and Filled Teeth (DMFT) score. Based on previous research, serological tests (detection of H. pylori antibodies) were performed to determine the presence of H. pylori in order to minimize discomfort and avoid the bias that occasionally arises with more intrusive gut collection methods. THE AIM: of this study is to provide a significant contribution to the present literature with genuine potential to influence public health policies and dental care programs targeted at reducing health inequities in the region, provided that the study is carefully planned and some innovative data management is done. RESULTS: This result essentially shows that additional interdisciplinary research is required to adequately map out the cause-and-effect relationship between H. pylori and dental caries. Curiously, it also supports previous research that suggested this bacterium might contribute to oral disorders, supporting the notion that a comprehensive, multimodal strategy is required to address oral and general health concerns. By lowering the rate of dental decay, local, community-driven initiatives to enhance oral hygiene may hold the key to minimizing the effects of H. pylori. The study emphasizes that reducing the disparities in oral health observed in Tripoli may depend on making dental treatment more accessible, particularly for those from lower-income backgrounds. Conclusion: An infection with Helicobacter pylori is substantially linked to a higher prevalence of dental caries. Both dental decay and H. pylori infection are significantly influenced by age, with poorer oral health being seen in older people. Infrequent dental checkups and poor oral hygiene are two factors that raise the DMFT index. People with diabetes, high blood pressure, and acidity have far worse tooth health. Dental caries is significantly influenced by economic level, with slightly worse outcomes for those with greater incomes. The DMFT score and H. pylori prevalence do not seem to be significantly impacted by smoking or gender. This study confirms our presumptions between H. pylori and dental cavities while highlighting the critical necessity for integrated healthcare systems. Keywords: Dental Caries, Helicobacter Pylori, Gastritis, Tripoli, Libya.

Abstract Background:- Depakine (valproic acid) is an antiepileptic medication that is commonly used as a first‑line treatment for a variety of seizures in both adults and children. However, it can result in testicular toxicity by increasing oxida tive stress inflammation. Melatonin (MLT) has antioxidant, anti‑inflammatory, and anti‑apoptotic potential. Therefore, the present study investigated the impact of MLT on depakine‑induced testicular damage in rats.

The World Health Organization declared the COVID-19 outbreak to be a global pandemic in March 2020, also the COVID-19 pandemic has caused drastic changes across the globe, affecting all areas of life. However, the pandemic cannot be ended overnight and more social distancing and other self-care measures are needed to protect our Libyan community. Therefore, people´s awareness, knowledge, attitudes, and appropriate behaviors are instrumental to containing the pandemic. This study aims to create a questionnaire to determine the Libyan society's environmental awareness of COVID-19

Shale gas is a form of natural gas found trapped within shale formations. Shale gas is vastly distributed around the world. Recently, there has been increasing interest in exploiting shale gas for energy production. As a source of energy, many countries started to investigate their shale gas potential. To understand the public’s views on the extraction of shale gas and its long and short-term health and environmental consequences, this study was undertaken to recognize Ghadames region residents’ awareness of the environmental and health effects resulting from the extraction of shale gas in their areas. Ghadames region is located in northwest Libya, where a potential deposit of shale gas is spotted; therefore, it was perfect as a study area. A population survey from a random sample (N=251) drawn from local residents; it was designed to detect the public opinion of the most likely adverse direct and indirect health and environmental outcomes as a result of fracking; the possible benefits were also included as well. The results show that the majority of respondents support shale gas extraction in their hometown, no matter how the consequences will affect their health and environment, about 70% of the respondents show support for shale gas exploitation

Shale gas and shale oil have rapidly revolutionized the energy supply and security landscape in various parts of the world. As Libya has a notable deposit of shale gas, this study sought to gain insight into the public perception of potential health and environmental impacts of a hydraulic fracturing and its associated controversies. It also sought to determine whether there is a need for public awareness of the science and economics associated with hydraulic fracturing. In this study, 304 local residents in Zawia city in the north west of Libya were surveyed on their views on the exploration of shale gas. The purpose of the survey is to understand the factors that influence local residents‖ support or opposition to the use of shale gas, such as energy independence, environmental consciousness, and risk/benefit perceptions. The results show that the respondents are generally supportive of shale gas extraction, the study show that 52% of the respondents express support or strong support for shale gas exploitation. Both this and future studies have the potential to improve public discourse and decision-making around the development of unconventional gas and oil industries.

Abstract

Background: Cutaneous Leishmaniasis (CL) is a disease transmitted by infected sandflies in tropical and subtropical areas of the world. Over 12 million people are infected with CL, with 90% of cases occurring in Afghanistan, Iran, Algeria, Brazil, Peru, Saudi Arabia, Syria, and Libya. CL causes localized skin lesions that may disappear without treatment or persist, causing severe tissue damage, permanent disfigurement, and serious disability. Physical methods such as cryotherapy, heat therapy, photodynamic therapy, laser, and radiofrequency have shown promising results in treating CL. However, none of the suggested treatments are universally effective, and many of them are associated with serious local and systemic side effects. Early diagnosis and treatment can cure the disease, prevent psychological impact, and help in the prevention of transmission. Different therapeutic modalities (monotherapy or combined therapy) were used in patient treatment, with different approaches planned according to the severity and duration of the condition.

Patients and methods: The study was conducted on 60 Patients who were divided into three groups: 20 patients treated with dapsone tab 100mg once daily for one month and 20 with radiofrequency, the third group, 20 Patients were treated with a combination of dapsone tab 100mg and radiofrequency. Treatment sessions were scheduled 1 session every 2 weeks. Statistical analysis was carried out using SPSS software version 18 (USA).

Results and Discussion: Patients underwent weekly physical exams to evaluate clinical response and adverse effects. Clinical response was classified as complete, good, or poor. Data were analyzed using one-way NOVA, Turkey's test, chisquare test, ANOVA, and Fischer's exact test. There was no significant difference among studied groups regarding demographic data. Patients treated with dapsone and radiofrequency were significantly more cleared in 1-2 months than those treated with radiofrequency alone. Conclusion: Patients had weekly physical exams to evaluate the clinical response and adverse effects. Clinical response was classified as complete, good, or poor. Data was analyzed using various tests. Dapsone and radiofrequency-treated patients cleared more in 1-2 months.

Keywords: Cutaneous leishmaniasis; Dapsone; Radiofrequency; Aalgabal algarby; Libya

This study investigated the challenges facing 4^th year Health Sciences College students in Gharyan University in translating/ learning English medical terms into Arabic. Two types of instruments were used to collect quantitative data needed for answering the research questions. The study participants were asked to translate and analyse some given medical English terms into Arabic, and via a questionnaire items they were asked to select the strategies they used to translate and analyse the given terms. The results revealed that many of the participants failed to translate the given medical terms into Arabic and most of them failed to analyse the terms into their individual segments. The analysed questionnaire data showed that most of the participants prefer to use bilingual dictionaries in translating the given terms. Explicit teaching of the medical terms translation with the focus on the meaning of the affixes and the roots of the terms may help in achieving correct translation of any English medical terms into Arabic language.  

Key words: challenges, medical terms, quantitative data, questionnaire.